Accessory Proteins Stabilize the Acceptor Complex for Synaptobrevin, the 1:1 Syntaxin/SNAP-25 Complex

Accessory proteins stabilize the acceptor complex for synaptobrevin, the 1:1 syntaxin/SNAP-25 complex.

Syntaxin/SNAP-25 interactions precede assembly of the ternary SNARE complex that is essential for neurotransmitter release. This binary complex has been difficult to characterize by bulk methods because of the prevalence of a 2:1 dead-end species. Here, using single-molecule fluorescence, we find the structure of the 1:1 syntaxin/SNAP-25 binary complex is variable, with states changing on the s...

-dependent Interaction with the Synaptobrevin–Synaptophysin Complex

Brain myosin V is a member of a widely distributed class of unconventional myosins that may be of central importance to organelle trafficking in all eukaryotic cells. Molecular constituents that target this molecular motor to organelles have not been previously identified. Using a combination of immunopurification, extraction, cross-linking, and coprecipitation assays, we demonstrate that the t...

The Quinone Iron Acceptor Complex

A coiled coil trigger site is essential for rapid binding of synaptobrevin to the SNARE acceptor complex.

Exocytosis from synaptic vesicles is driven by stepwise formation of a tight alpha-helical complex between the fusing membranes. The complex is composed of the three SNAREs: synaptobrevin 2, SNAP-25, and syntaxin 1a. An important step in complex formation is fast binding of vesicular synaptobrevin to the preformed syntaxin 1.SNAP-25 dimer. Exactly how this step relates to neurotransmitter relea...

Strengthening the Hospital Complex

بیمارستانها باید در کنار درمان افراد بیمار، در زمین برنامه ریزی، هماهنگی، اجرا، نظارت و کنترل مراقبت های اولیه بهداشتی در مناطق مربوط به خودشان نیز مشارکت داشته باشند. بیمارستانها می توانند مسئولیت تخصیص و کنترل منابع را در سراسر منطقه با توجه به نحوه سازان دهی سرویسهای بهداشتی در کشور به عهده داشته باشند.